pPD540 Reporter Template v3
(Plasmid #138750)

• Purpose: (Empty Backbone) Template for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce non-palindromic sticky ends.
• Depositing Lab: Joshua Leonard
• Publication: Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.

Backbone

• Vector backbone: pPD540 Reporter Template v3
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Supplemental Documents:
  • pPD540 Reporter Template v3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compatible with BsaI/BpiI Golden Gate system. As of February 2020, this is the preferred template for COMET reporters, as it uses non-palindromic sticky ends for promoter insertion, to reduce the risk of multiple insertions. To insert promoter, use annealed primers or PCR product in a Golden Gate Reaction with BsaI. Leonard Lab plasmid reference number: L1270