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Addgene

pLenti-CMV-Blast-PIP-FUCCI
(Plasmid #138715)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138715 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMV Blast DEST (706–1)
  • Backbone size w/o insert (bp) 9331
  • Total vector size (bp) 9692
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PIP-FUCCI
  • Alt name
    PIP-NLS-mVenus-P2A-mCherry-Gem(1-110)
  • Species
    Synthetic
  • Insert Size (bp)
    1992
  • Promoter CMV
  • Tags / Fusion Proteins
    • mVenus (C terminal on insert)
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV promoter primer (5'-ATG GGC GGT AGG CGT GTA CG)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CMV-Blast-PIP-FUCCI was a gift from Jean Cook (Addgene plasmid # 138715 ; http://n2t.net/addgene:138715 ; RRID:Addgene_138715)

  • For your References section:

    Accurate delineation of cell cycle phase transitions in living cells with PIP-FUCCI. Grant GD, Kedziora KM, Limas JC, Cook JG, Purvis JE. Cell Cycle. 2018;17(21-22):2496-2516. doi: 10.1080/15384101.2018.1547001. 10.1080/15384101.2018.1547001 PubMed 30421640
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