pJMD5
(Plasmid
#138652)
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Purpose(Empty Backbone) phiBT1 integrating Streptomyces expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 138652 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJMD5
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Vector typeBacterial Expression
- Promoter PermE* variant sequence from pDA1652
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Selectable markersthiostrepton
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJMD5 was a gift from Joshua Blodgett (Addgene plasmid # 138652 ; http://n2t.net/addgene:138652 ; RRID:Addgene_138652) -
For your References section:
Construction of a new integrating vector from actinophage varphiOZJ and its use in multiplex Streptomyces transformation. Ko B, D'Alessandro J, Douangkeomany L, Stumpf S, deButts A, Blodgett J. J Ind Microbiol Biotechnol. 2019 Nov 8. pii: 10.1007/s10295-019-02246-7. doi: 10.1007/s10295-019-02246-7. 10.1007/s10295-019-02246-7 PubMed 31705217