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Addgene

HA-RNF126 C231A
(Plasmid #138648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-HA2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNF126
  • Species
    M. musculus (mouse)
  • Mutation
    C231A
  • Entrez Gene
    RNF126
  • Tag / Fusion Protein
    • HA-HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-RNF126 C231A was a gift from Jane McGlade (Addgene plasmid # 138648 ; http://n2t.net/addgene:138648 ; RRID:Addgene_138648)

  • For your References section:

    The ubiquitin ligase RNF126 regulates the retrograde sorting of the cation-independent mannose 6-phosphate receptor. Smith CJ, McGlade CJ. Exp Cell Res. 2014 Jan 15;320(2):219-32. doi: 10.1016/j.yexcr.2013.11.013. Epub 2013 Nov 23. 10.1016/j.yexcr.2013.11.013 PubMed 24275455
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