GST-RNF126 C13,16A
(Plasmid
#138645)
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PurposeBacterial expression of GST-RNF126 ZnF mutant (C13,16A) fusion protein
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-4T3
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRNF126
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SpeciesM. musculus (mouse)
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MutationC13A, C16A
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Entrez GeneRNF126
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (unknown if destroyed)
- 3′ cloning site Xho1 (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Double Cys to Ala mutation os Zinc finger domain of mouse RNF126; made by site directed mutagenesis
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-RNF126 C13,16A was a gift from Jane McGlade (Addgene plasmid # 138645 ; http://n2t.net/addgene:138645 ; RRID:Addgene_138645) -
For your References section:
The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor. Smith CJ, Berry DM, McGlade CJ. J Cell Sci. 2013 Mar 15;126(Pt 6):1366-80. doi: 10.1242/jcs.116129. Epub 2013 Feb 15. 10.1242/jcs.116129 PubMed 23418353
