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Addgene

GST-RNF126 C231A
(Plasmid #138644)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138644 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T3
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNF126
  • Species
    M. musculus (mouse)
  • Mutation
    C231A
  • Entrez Gene
    RNF126
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-RNF126 C231A was a gift from Jane McGlade (Addgene plasmid # 138644 ; http://n2t.net/addgene:138644 ; RRID:Addgene_138644)

  • For your References section:

    The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor. Smith CJ, Berry DM, McGlade CJ. J Cell Sci. 2013 Mar 15;126(Pt 6):1366-80. doi: 10.1242/jcs.116129. Epub 2013 Feb 15. 10.1242/jcs.116129 PubMed 23418353
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