RCAS (B) cPitx1 (CT#69)
(Plasmid
#13864)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRCASBP (B)
- Backbone size w/o insert (bp) 11600
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Vector typeRetroviral ; avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepitx1
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SpeciesG. gallus (chicken)
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Insert Size (bp)900
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Entrez GenePITX1 (a.k.a. PTX1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The entire open reading frame of chick Pitx1 was cloned into the RCAS plasmid using conventional methods
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS (B) cPitx1 (CT#69) was a gift from Cliff Tabin (Addgene plasmid # 13864 ; http://n2t.net/addgene:13864 ; RRID:Addgene_13864) -
For your References section:
Role of Pitx1 upstream of Tbx4 in specification of hindlimb identity. Logan M, Tabin CJ. Science. 1999 Mar 12. 283(5408):1736-9. 10.1126/science.283.5408.1736 PubMed 10073939