RCAS (A) Caronte (CT#382)
(Plasmid
#13862)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRCAS BP (A)
- Backbone size w/o insert (bp) 11600
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Vector typeRetroviral ; avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaronte
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Alt namecar
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SpeciesG. gallus (chicken)
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Insert Size (bp)900
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Entrez GeneCER1 (a.k.a. CAR)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
~600 bp IRES plus ~900 bp of Caronte. IRES (NcoI) Caronte (EcoRI) -> cloned into ClaI site of RCAS.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS (A) Caronte (CT#382) was a gift from Cliff Tabin (Addgene plasmid # 13862 ; http://n2t.net/addgene:13862 ; RRID:Addgene_13862) -
For your References section:
Antagonistic signaling by Caronte, a novel Cerberus-related gene, establishes left-right asymmetric gene expression. Yokouchi Y, Vogan KJ, Pearse RV, Tabin CJ. Cell. 1999 Sep 3. 98(5):573-83. 10.1016/S0092-8674(00)80045-8 PubMed 10490097
Map uploaded by the depositor.
