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Addgene

RCAS (A) Caronte (CT#382)
(Plasmid #13862)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13862 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    RCAS BP (A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Caronte
  • Alt name
    car
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    900
  • Entrez Gene
    CER1 (a.k.a. CAR)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

~600 bp IRES plus ~900 bp of Caronte. IRES (NcoI) Caronte (EcoRI) -> cloned into ClaI site of RCAS.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS (A) Caronte (CT#382) was a gift from Cliff Tabin (Addgene plasmid # 13862 ; http://n2t.net/addgene:13862 ; RRID:Addgene_13862)
  • For your References section:

    Antagonistic signaling by Caronte, a novel Cerberus-related gene, establishes left-right asymmetric gene expression. Yokouchi Y, Vogan KJ, Pearse RV, Tabin CJ. Cell. 1999 Sep 3. 98(5):573-83. 10.1016/S0092-8674(00)80045-8 PubMed 10490097