pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato
(Plasmid #138610)

• Purpose: Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone
• Depositing Lab: Fei Chen
• Publication: Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 10.1038/s41587-019-0331-8.

Backbone

• Vector backbone: pcDNA3.1(+)
• Total vector size (bp): 10336
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Hyperactive AID
• Alt name: pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase G645AQ744R-UGI - T2A - tdTomato
• Insert Size (bp): 585

Gene/Insert 2

• Gene/Insert name: T7 RNA Polymerase
• Insert Size (bp): 2646

Gene/Insert 3

• Gene/Insert name: tdTomato
• Insert Size (bp): 1428

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Hyperactive AID was cloned from pGH335_MS2-AID*Δ-Hygro obtained from Michael Bassik (Addgene plasmid # 85406)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry