pcDNA3.1_GP1Fc Cl13
(Plasmid #138599)

• Purpose: Expresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion)
• Depositing Lab: Annette Oxenius
• Publication: Krautler et al Cell Rep. 2020 Jan 28;30(4):997-1012.e6. doi: 10.1016/j.celrep.2019.12.088.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: -
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LCMV GP1-Fc
• Species: H. sapiens (human); Lymphocytic Choriomeningitis Virus (LCMV) Strain Clone 13
• Insert Size (bp): 1497
• Promoter: CMV
• Tag / Fusion Protein:
  • Fc fusion protein

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CAG AGC TCT CTG GCT AAC TA
• 3′ sequencing primer: CTT CCA GGG TCA AGG AAG

Resource Information

• Supplemental Documents:
  • pcDNA3.1_GP1Fc.gbk
• A portion of this plasmid was derived from a plasmid made by: Dr. Bruno Eschli

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry