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pcDNA3.1_GP1Fc Cl13
(Plasmid #138599)

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This material is available to academics and nonprofits only. Availability may be limited outside the U.S. Please log in for more information.
Item Catalog # Description Quantity Price (USD)
Plasmid 138599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    -
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LCMV GP1-Fc
  • Species
    H. sapiens (human); Lymphocytic Choriomeningitis Virus (LCMV) Strain Clone 13
  • Insert Size (bp)
    1497
  • Promoter CMV
  • Tag / Fusion Protein
    • Fc fusion protein

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CAG AGC TCT CTG GCT AAC TA
  • 3′ sequencing primer CTT CCA GGG TCA AGG AAG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Dr. Bruno Eschli

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_GP1Fc Cl13 was a gift from Annette Oxenius (Addgene plasmid # 138599 ; http://n2t.net/addgene:138599 ; RRID:Addgene_138599)

  • For your References section:

    Quantitative and Qualitative Analysis of Humoral Immunity Reveals Continued and Personalized Evolution in Chronic Viral Infection. Krautler NJ, Yermanos A, Pedrioli A, Welten SPM, Lorge D, Greczmiel U, Bartsch I, Scheuermann J, Kiefer JD, Eyer K, Menzel U, Greiff V, Neri D, Stadler T, Reddy ST, Oxenius A. Cell Rep. 2020 Jan 28;30(4):997-1012.e6. doi: 10.1016/j.celrep.2019.12.088. 10.1016/j.celrep.2019.12.088 PubMed 31995768
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