pWDY1222
(Plasmid
#138592)
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PurposeExpresses NatL2-K418A in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 138592 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-28a(+)
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6622
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namephenylacetate-coenzyme A ligase
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Alt nameNatL2-K418A
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SpeciesStreptomyces sp. Tu 6176
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Mutationchanged Lysine 418 to Alanine
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GenBank IDCEK42825.1
- Promoter T7
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Tag
/ Fusion Protein
- 6-His (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TGCCGCGCGGCAGCCATATGATGTCGCGCTCGCGTCCGGAACTTGGTGACTG
- 3′ sequencing primer GGTGGTGCTCGAGTGCGGCCGCAAGCTTTCAGAAGTACATGACCGCCTTGTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWDY1222 was a gift from James Naismith (Addgene plasmid # 138592 ; http://n2t.net/addgene:138592 ; RRID:Addgene_138592) -
For your References section:
The biosynthetic pathway of the benzoxazole in nataxazole proceeds via an unstable ester and has synthetic utility. Song H, Rao C, Deng Z, Yi Y, Naismith JH. Angew Chem Int Ed Engl. 2020 Jan 5. doi: 10.1002/anie.201915685. 10.1002/anie.201915685 PubMed 31903677