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Addgene

pWDY1222
(Plasmid #138592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6622
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    phenylacetate-coenzyme A ligase
  • Alt name
    NatL2-K418A
  • Species
    Streptomyces sp. Tu 6176
  • Mutation
    changed Lysine 418 to Alanine
  • GenBank ID
    CEK42825.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6-His (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGCCGCGCGGCAGCCATATGATGTCGCGCTCGCGTCCGGAACTTGGTGACTG
  • 3′ sequencing primer GGTGGTGCTCGAGTGCGGCCGCAAGCTTTCAGAAGTACATGACCGCCTTGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWDY1222 was a gift from James Naismith (Addgene plasmid # 138592 ; http://n2t.net/addgene:138592 ; RRID:Addgene_138592)

  • For your References section:

    The biosynthetic pathway of the benzoxazole in nataxazole proceeds via an unstable ester and has synthetic utility. Song H, Rao C, Deng Z, Yi Y, Naismith JH. Angew Chem Int Ed Engl. 2020 Jan 5. doi: 10.1002/anie.201915685. 10.1002/anie.201915685 PubMed 31903677
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