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pET30b_T7Sav
(Plasmid #138588)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138588 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30b
  • Backbone manufacturer
    Novagen
  • Total vector size (bp) 5761
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Glucose can be added to growth medium to reduce leaky expression
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    T7-tagged streptavidin variant with native C-terminus
  • Alt name
    T7SAV
  • Species
    Synthetic
  • Insert Size (bp)
    483
  • Promoter PT7
  • Tag / Fusion Protein
    • T7-tag (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ATGCGTCCGGCGTAGA
  • 3′ sequencing primer TGCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30b_T7Sav was a gift from Markus Jeschek (Addgene plasmid # 138588 ; http://n2t.net/addgene:138588 ; RRID:Addgene_138588)

  • For your References section:

    Biotin-independent strains of Escherichia coli for enhanced streptavidin production. Jeschek M, Bahls MO, Schneider V, Marliere P, Ward TR, Panke S. Metab Eng. 2017 Mar;40:33-40. doi: 10.1016/j.ymben.2016.12.013. Epub 2017 Jan 3. 10.1016/j.ymben.2016.12.013 PubMed 28062280
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