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Addgene

pLVX ALIX-GFP S718/721A
(Plasmid #138583)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138583 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Backbone size w/o insert (bp) 7769
  • Total vector size (bp) 11183
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ALIX
  • Alt name
    PDCD6IP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3414
  • Mutation
    changed serines 718 and 721 to alanines
  • GenBank ID
    HGNC:8766 OMIM: 608074
  • Entrez Gene
    PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
  • Promoter TREtight
  • Tags / Fusion Proteins
    • h30 (C terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gtcgaggtaggcgtgtacgg
  • 3′ sequencing primer gaggccagaggccacttgtgtag
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX ALIX-GFP S718/721A was a gift from Wesley Sundquist (Addgene plasmid # 138583 ; http://n2t.net/addgene:138583 ; RRID:Addgene_138583)
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