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pGL4.24-Cd36-enhancer5
(Plasmid #138577)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138577 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.24
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4411
  • Total vector size (bp) 5755
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cd36 Promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1344
  • GenBank ID
    NC_000071.6
  • Entrez Gene
    Cd36 (a.k.a. FAT, GPIV, Scarb3)
  • Promoter Minimal promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer TGGCTTTACCAACAGTACCGGA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.24-Cd36-enhancer5 was a gift from Lora Hooper (Addgene plasmid # 138577 ; http://n2t.net/addgene:138577 ; RRID:Addgene_138577)

  • For your References section:

    The intestinal microbiota programs diurnal rhythms in host metabolism through histone deacetylase 3. Kuang Z, Wang Y, Li Y, Ye C, Ruhn KA, Behrendt CL, Olson EN, Hooper LV. Science. 2019 Sep 27;365(6460):1428-1434. doi: 10.1126/science.aaw3134. 10.1126/science.aaw3134 PubMed 31604271
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