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Addgene

pCRISPR-HOT-P2A-Clover-BlastR
(Plasmid #138568)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P2A-clover-BlastR

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPR-HOT-P2A-Clover-BlastR was a gift from Hans Clevers (Addgene plasmid # 138568 ; http://n2t.net/addgene:138568 ; RRID:Addgene_138568)

  • For your References section:

    Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing. Artegiani B, Hendriks D, Beumer J, Kok R, Zheng X, Joore I, Chuva de Sousa Lopes S, van Zon J, Tans S, Clevers H. Nat Cell Biol. 2020 Mar;22(3):321-331. doi: 10.1038/s41556-020-0472-5. Epub 2020 Mar 2. 10.1038/s41556-020-0472-5 PubMed 32123335
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