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Addgene

pIVT-myc-T58A-S62D
(Plasmid #138540)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138540 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIVT
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 3059
  • Total vector size (bp) 4379
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myelocytomatosis oncogene T58A S62D
  • Alt name
    Myc T58A S62D
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1320
  • Mutation
    Threonine 58 changed to alanine and serine 62 changed to aspartic acid
  • GenBank ID
    NM_001177352.1
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dharmacon

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVT-myc-T58A-S62D was a gift from Carmen Williams (Addgene plasmid # 138540 ; http://n2t.net/addgene:138540 ; RRID:Addgene_138540)
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