Skip to main content
Addgene

pGEMHE-TNKS-2-M1054V
(Plasmid #138538)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138538 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEMHE
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 3064
  • Total vector size (bp) 6601
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tankyrase 2
  • Alt name
    TANKS2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3537
  • Mutation
    Changed methionine 1054 to valine
  • GenBank ID
    NM_025235.4
  • Entrez Gene
    TNKS2 (a.k.a. ARTD6, PARP-5b, PARP-5c, PARP5B, PARP5C, TANK2, TNKL, pART6)
  • Promoter T7
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Age1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene Cat#34692

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMHE-TNKS-2-M1054V was a gift from Carmen Williams (Addgene plasmid # 138538 ; http://n2t.net/addgene:138538 ; RRID:Addgene_138538)
Related items: