pFUW-TetO-mCherry-cNLS
(Plasmid #138531)

• Purpose: Lentiviral inducible expression of mCherry with nuclear localization signal
• Depositing Labs: Christopher Grigsby, Ana Teixeira
• Publication: Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2978-2986. doi: 10.1073/pnas.1919600117. Epub 2020 Jan 27.

Backbone

• Vector backbone: FUW-tetO-MCS
• Backbone manufacturer: Stefano Piccolo (Addgene plasmid # 84008)
• Backbone size w/o insert (bp): 8477
• Total vector size (bp): 9315
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 844
• GenBank ID: AY678264.1
• Promoter: TRE-CMV
• Tag / Fusion Protein:
  • 3x SV40 cNLS (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry