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Addgene

pHLS-EF1a-FRB-SpCas9-A
(Plasmid #138477)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138477 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHLS
  • Backbone manufacturer
    Hotta lab
  • Backbone size w/o insert (bp) 4762
  • Total vector size (bp) 9007
  • Modifications to backbone
    pHL vector with SV40 replication origin.
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9, human codon optimized
  • Alt name
    CRISPR-Cas9
  • Species
    Streptococcus pyogenes
  • Tags / Fusion Proteins
    • FRB (N terminal on insert)
    • 3x HA tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHLS-EF1a-FRB-SpCas9-A was a gift from Akitsu Hotta (Addgene plasmid # 138477 ; http://n2t.net/addgene:138477 ; RRID:Addgene_138477)

  • For your References section:

    Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping. Gee P, Lung MSY, Okuzaki Y, Sasakawa N, Iguchi T, Makita Y, Hozumi H, Miura Y, Yang LF, Iwasaki M, Wang XH, Waller MA, Shirai N, Abe YO, Fujita Y, Watanabe K, Kagita A, Iwabuchi KA, Yasuda M, Xu H, Noda T, Komano J, Sakurai H, Inukai N, Hotta A. Nat Commun. 2020 Mar 13;11(1):1334. doi: 10.1038/s41467-020-14957-y. 10.1038/s41467-020-14957-y PubMed 32170079
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