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PurposeEnables high-efficiency recombineering in P. aeruginosa
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 138475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA258
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 7620
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)MG1655
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namePapRecT
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SpeciesP. aeruginosa
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Insert Size (bp)747
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GenBank IDWP_003088368
Gene/Insert 2
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Gene/Insert namemutL E36K
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Alt nameDominant-Negative MutL
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SpeciesP. aeruginosa
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Insert Size (bp)1899
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MutationE36K
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GenBank IDWP_128670263.1
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
bioRxiv 10.1101/2020.01.14.906594
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pORTMAGE-Pa1 was a gift from George Church (Addgene plasmid # 138475 ; http://n2t.net/addgene:138475 ; RRID:Addgene_138475) -
For your References section:
Improved bacterial recombineering by parallelized protein discovery. Wannier TM, Nyerges A, Kuchwara HM, Czikkely M, Balogh D, Filsinger GT, Borders NC, Gregg CJ, Lajoie MJ, Rios X, Pal C, Church GM. Proc Natl Acad Sci U S A. 2020 Jun 16;117(24):13689-13698. doi: 10.1073/pnas.2001588117. Epub 2020 May 28. 10.1073/pnas.2001588117 PubMed 32467157