piB-GFP
(Plasmid #13844)

• Depositing Lab: Ting Wu
• Publication: Bateman et al Genetics. 2006 Jun . 173(2):769-77.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 3000
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TATA-GFP-SV40
• Alt name: GFP
• Insert Size (bp): 2600

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (not destroyed)
• 3′ cloning site: See map (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: attB from Michele Calos, Stanford
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

piB-GFP is a donor vector that contains an hsp70 TATA box fused to an enhanced GFP open reading frame and an SV40 polyA tail. This gene construct is flanked by inverted attB sites in the vector pBluescript. piB-GFP is a useful vector for cloning a gene of interest into a donor cassette; the GFP gene can be excised and new sequences can be inserted between the inverted attB sites using BamHI, SalI, or HindIII.

*One note on preparing this vector: we had difficulty growing piB-GFP in a larger 25 ml culture for midiprepping, but there were no problems in smaller 3 ml cultures suitable for minipreps. For all of our GFP injections, we used mini-prepped piB-GFP that was further purified on a pcr-purification column to remove RNAse contaminants.