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Addgene

pUASTP2
(Plasmid #13843)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13843 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mini-white
  • Alt name
    white
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    4000
  • Entrez Gene
    w (a.k.a. Dmel_CG2759, BACN33B1.1, CG2759, DMWHITE, DmWhite, Dmel\CG2759, EG:BACN33B1.1, W, White, c23, e(g), m(g), mini-white, mw, w(AT)[[13]])

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attP (not destroyed)
  • 3′ cloning site attP (not destroyed)
  • 5′ sequencing primer Pry1
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    attP from Michele Calos, Stanford
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pUASTP2 contains a P-element target for RMCE, the mini-white gene flanked by inverted attP sites. This target cassette was contructed in the parent vector pUAST. This vector can be used to generate target lines by standard P element transgenesis.

Please note that the UAS binding sites and hsp70 promoter of pUAST are outside of the exchange cassette in this vector - i.e. following RMCE, these elements will remain flanking the cassette.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASTP2 was a gift from Ting Wu (Addgene plasmid # 13843 ; http://n2t.net/addgene:13843 ; RRID:Addgene_13843)
  • For your References section:

    Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Bateman JR, Lee AM, Wu CT. Genetics. 2006 Jun . 173(2):769-77. 10.1534/genetics.106.056945 PubMed 16547094