pUASTP2.1
(Plasmid #13842)

• Depositing Lab: Ting Wu
• Publication: Bateman et al Genetics. 2006 Jun . 173(2):769-77.

Backbone

• Vector backbone: pUAST
• Backbone size w/o insert (bp): 4300
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mini-white
• Alt name: white
• Species: D. melanogaster (fly)
• Insert Size (bp): 4000
• Entrez Gene: w (a.k.a. Dmel_CG2759, BACN33B1.1, CG2759, DMWHITE, DmWhite, Dmel\CG2759, EG:BACN33B1.1, W, White, c23, e(g), m(g), mini-white, mw, w(AT)[[13]])

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attP (not destroyed)
• 3′ cloning site: attP (not destroyed)
• 5′ sequencing primer: Pry1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: attP from Michele Calos, Stanford

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pUASTP2.1 contains a P-element target for RMCE, the mini-white gene flanked by inverted attP sites. It is a derivative of PUASTP2 in which the UAS and TATA sequences flanking the cassette have been removed. (Specifically, PUASTP2 was digested with BamHI and StuI to remove UAS-TATA-attP, and an attP-containing BamHI-EcoRV fragment from pTA-attP was inserted). This vector can be used to generate target lines by standard P element transgenesis.