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CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1
(Plasmid #138419)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138419 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-EF1a-zsGreen1
  • Backbone manufacturer
    Takara Bio
  • Vector type
    Lentiviral
  • Selectable markers
    zsGreen1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4425
  • Promoter EF1a
  • Tag / Fusion Protein
    • BioTAP-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1 was a gift from Jian Xu (Addgene plasmid # 138419 ; http://n2t.net/addgene:138419 ; RRID:Addgene_138419)

  • For your References section:

    Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9. Liu X, Chen Y, Zhang Y, Liu Y, Liu N, Botten GA, Cao H, Orkin SH, Zhang MQ, Xu J. Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w. 10.1186/s13059-020-01973-w PubMed 32138752
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