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PurposeCAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 138418 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX-EF1a-zsGreen1
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Backbone manufacturerTakara Bio
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Vector typeLentiviral
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Selectable markerszsGreen1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedCas9
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SpeciesStreptococcus pyogenes
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Insert Size (bp)4401
- Promoter EF1a
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Tag
/ Fusion Protein
- BioTAP-tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1 was a gift from Jian Xu (Addgene plasmid # 138418 ; http://n2t.net/addgene:138418 ; RRID:Addgene_138418) -
For your References section:
Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9. Liu X, Chen Y, Zhang Y, Liu Y, Liu N, Botten GA, Cao H, Orkin SH, Zhang MQ, Xu J. Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w. 10.1186/s13059-020-01973-w PubMed 32138752