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Addgene

pKH3-human RSK1
(Plasmid #13841)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13841 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKH3
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RSK1
  • Species
    H. sapiens (human)
  • Entrez Gene
    RPS6KA1 (a.k.a. HU-1, MAPKAPK1, MAPKAPK1A, RSK, RSK1, p90Rsk)
  • Tag / Fusion Protein
    • 3x HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKH3-human RSK1 was a gift from John Blenis (Addgene plasmid # 13841 ; http://n2t.net/addgene:13841 ; RRID:Addgene_13841)

  • For your References section:

    Characterization of regulatory events associated with membrane targeting of p90 ribosomal S6 kinase 1. Richards SA, Dreisbach VC, Murphy LO, Blenis J. Mol Cell Biol. 2001 Nov . 21(21):7470-80. 10.1128/MCB.21.21.7470-7480.2001 PubMed 11585927
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