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Addgene

pBFv-nosP-Cas9
(Plasmid #138402)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138402 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescriptII SK (+)
  • Vector type
    Insect Expression
  • Selectable markers
    vermilion (expression very weak)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-NLS

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBFv-nosP-Cas9 was a gift from Shu Kondo (Addgene plasmid # 138402 ; http://n2t.net/addgene:138402 ; RRID:Addgene_138402)

  • For your References section:

    Highly improved gene targeting by germline-specific Cas9 expression in Drosophila. Kondo S, Ueda R. Genetics. 2013 Nov;195(3):715-21. doi: 10.1534/genetics.113.156737. Epub 2013 Sep 3. 10.1534/genetics.113.156737 PubMed 24002648
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