EF2451_pET21a(+)
(Plasmid
#13840)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-21a(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5363
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePPAT
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Alt namephosphopantetheine adenylyltransferase
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Alt nameEF 2451
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Alt nameEf2451
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SpeciesEnterococcus faecalis
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Insert Size (bp)492
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GenBank IDNC_004669
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EF2451_pET21a(+) was a gift from SeWon Suh (Addgene plasmid # 13840 ; http://n2t.net/addgene:13840 ; RRID:Addgene_13840) -
For your References section:
Overexpression, crystallization and preliminary X-ray crystallographic analysis of phosphopantetheine adenylyltransferase from Enterococcus faecalis. Kang JY, Lee HH, Yoon HJ, Kim HS, Suh SW. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Nov 1. 62(Pt 11):1131-3. 10.1107/S1744309106041108 PubMed 17077496
