TA0474_pET21a(+)
(Plasmid #13839)

• Depositing Lab: SeWon Suh
• Publication: Kang et al Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Nov 1. 62(Pt 11):1147-9.

Backbone

• Vector backbone: pET-21a(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5363
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: IS200 transposase
• Alt name: TA0474
• Alt name: transposase
• Alt name: Ta0474
• Species: Thermoplasma acidophilum
• Insert Size (bp): 411
• Mutation: See Depositor Comments Below
• GenBank ID: NC_002578
• Entrez Gene: Ta0474

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a D75N mutation in IS200 transposase. It is not known how this mutation affects plasmid function.