pPyCAG-Nanog-IP
(Plasmid #13838)

• Depositing Lab: Shinya Yamanaka
• Publication: Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Backbone

• Vector backbone: pPyCAG-gw-IP
• Backbone size w/o insert (bp): 6800
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nanog
• Species: M. musculus (mouse)
• Insert Size (bp): 900
• Mutation: None
• Entrez Gene: Nanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CAG-1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry