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p5E-161K enhancer-ETS-CORE-MUT
(Plasmid #138372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONRP4-P1R
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 2643
  • Total vector size (bp) 3634
  • Modifications to backbone
    none
  • Vector type
    Gateway compatible

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human 161K human enhancer with ETS bind sites mutated
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    991
  • Mutation
    ETS factor binding sites mutated
  • Promoter NONE

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was constructed to functionally validate ETS factor binding sites in the 161K human enhancer.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-161K enhancer-ETS-CORE-MUT was a gift from Nathan Lawson (Addgene plasmid # 138372 ; http://n2t.net/addgene:138372 ; RRID:Addgene_138372)

  • For your References section:

    Genomic Characterization of Endothelial Enhancers Reveals a Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene Expression. Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, Lawson ND. Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075. 10.1161/CIRCRESAHA.119.316075 PubMed 32065070
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