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Addgene

p5E-161K enhancer
(Plasmid #138365)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138365 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONRP4-P1R
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 2643
  • Total vector size (bp) 3634
  • Modifications to backbone
    none
  • Vector type
    Gateway compatible

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    161k human enhancer
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    991
  • Mutation
    9 A bases deletion, that have no impact on the enhancer function

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was constructed to functionally validate the 161K enhancer in luciferase reporter assays.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-161K enhancer was a gift from Nathan Lawson (Addgene plasmid # 138365 ; http://n2t.net/addgene:138365 ; RRID:Addgene_138365)

  • For your References section:

    Genomic Characterization of Endothelial Enhancers Reveals a Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene Expression. Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, Lawson ND. Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075. 10.1161/CIRCRESAHA.119.316075 PubMed 32065070
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