pLenti-CMVSP6-nEGFP-SV40-PURO
(Plasmid #138364)

• Purpose: Lentiviral plasmid for nuclear EGFP expression driven by CMVSP6 and puromycin resistance under SV40 promoter for selection
• Depositing Lab: Nathan Lawson
• Publication: Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075.

Backbone

• Vector backbone: Lentiviral
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: nuclear EGFP
• Alt name: nEGFP
• Insert Size (bp): 795

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is used as a positive control for lentiviral infection, EGFP is visible if the cell transduction is successful.