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Addgene

pTV-Nanog Hyg
(Plasmid #13836)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13836 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression ; Beta-gal
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRES-Hyg-pA cassette flanked by Nanog homologous regions
  • Alt name
    Nanog
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    8000
  • Mutation
    None
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTV-Nanog Hyg was a gift from Shinya Yamanaka (Addgene plasmid # 13836 ; http://n2t.net/addgene:13836 ; RRID:Addgene_13836)

  • For your References section:

    The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S. Cell. 2003 May 30. 113(5):631-42. 10.1016/S0092-8674(03)00393-3 PubMed 12787504
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