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Addgene

pME-minP:luc2
(Plasmid #138354)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138354 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PDONR221
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 2545
  • Total vector size (bp) 4280
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Alt name
    Luc2
  • Insert Size (bp)
    1736
  • Promoter minimal promoter

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The luc2 insert in this plasmid is preceded by minimal promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-minP:luc2 was a gift from Nathan Lawson (Addgene plasmid # 138354 ; http://n2t.net/addgene:138354 ; RRID:Addgene_138354)

  • For your References section:

    Genomic Characterization of Endothelial Enhancers Reveals a Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene Expression. Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, Lawson ND. Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075. 10.1161/CIRCRESAHA.119.316075 PubMed 32065070
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