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Addgene

pCOM-CD63-4xM12-COM
(Plasmid #138351)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138351 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    CD63-pEGFP C2
  • Backbone manufacturer
    Addgene 62964
  • Backbone size w/o insert (bp) 3937
  • Total vector size (bp) 5351
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    COM-CD63-4xM12-COM fusion protein
  • Species
    Human and bacteriophage
  • Insert Size (bp)
    1404
  • Tag / Fusion Protein
    • COM-CD63-4xM12-COM, four copies of muscle homing M12 peptide is inserted into the extramembrane loop of CD63

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert sequence can be unstable during bacterial propagation. Restriction digests should be used to verify the full-length plasmid is present.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCOM-CD63-4xM12-COM was a gift from Baisong Lu (Addgene plasmid # 138351)

  • For your References section:

    Engineered extracellular vesicles as versatile ribonucleoprotein delivery vehicles for efficient and safe CRISPR genome editing. Yao X, Lyu P, Yoo K, Yadav MK, Singh R, Atala A, Lu B. J Extracell Vesicles. 2021 Mar;10(5):e12076. doi: 10.1002/jev2.12076. Epub 2021 Mar 16. 10.1002/jev2.12076 PubMed 33747370
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