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Addgene

pCMV-BE4-PpAPOBEC1
(Plasmid #138349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 3399
  • Total vector size (bp) 8958
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BE4-PpAPOBEC1
  • Species
    Synthetic
  • Insert Size (bp)
    5559
  • Promoter CMV
  • Tag / Fusion Protein
    • BP-NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-BE4-PpAPOBEC1 was a gift from Nicole Gaudelli (Addgene plasmid # 138349 ; http://n2t.net/addgene:138349 ; RRID:Addgene_138349)

  • For your References section:

    Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity. Yu Y, Leete TC, Born DA, Young L, Barrera LA, Lee SJ, Rees HA, Ciaramella G, Gaudelli NM. Nat Commun. 2020 Apr 28;11(1):2052. doi: 10.1038/s41467-020-15887-5. 10.1038/s41467-020-15887-5 PubMed 32345976
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