pCMV-BE4-PpAPOBEC1 H122A
(Plasmid
#138338)
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PurposeMammalian expression plasmid for BE4-PpABOBEC1H122A
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138338 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 3399
- Total vector size (bp) 8958
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBE4-PpAPOBEC1 H122A
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SpeciesSynthetic
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Insert Size (bp)5559
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MutationChange of His122 to Ala.
- Promoter CMV
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Tag
/ Fusion Protein
- BP-NLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-BE4-PpAPOBEC1 H122A was a gift from Nicole Gaudelli (Addgene plasmid # 138338 ; http://n2t.net/addgene:138338 ; RRID:Addgene_138338) -
For your References section:
Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity. Yu Y, Leete TC, Born DA, Young L, Barrera LA, Lee SJ, Rees HA, Ciaramella G, Gaudelli NM. Nat Commun. 2020 Apr 28;11(1):2052. doi: 10.1038/s41467-020-15887-5. 10.1038/s41467-020-15887-5 PubMed 32345976
