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Addgene

pMXs-EGFP-Rheb-HVR HRas-IP
(Plasmid #13833)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13833 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMX-EGFP-gw-IP
  • Backbone size w/o insert (bp) 6900
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rheb-HVR HRas
  • Alt name
    Rheb
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    700
  • Mutation
    N-terminal of Rheb and hyper variable region (HVR) of HRas are fused in frame
  • Entrez Gene
    Rheb (a.k.a. Rheb1)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-EGFP-Rheb-HVR HRas-IP was a gift from Shinya Yamanaka (Addgene plasmid # 13833 ; http://n2t.net/addgene:13833 ; RRID:Addgene_13833)
  • For your References section:

    Differential membrane localization of ERas and Rheb, two Ras-related proteins involved in the phosphatidylinositol 3-kinase/mTOR pathway. Takahashi K, Nakagawa M, Young SG, Yamanaka S. J Biol Chem. 2005 Sep 23. 280(38):32768-74. 10.1074/jbc.M506280200 PubMed 16046393