pMXs-EGFP-Rheb-HVR HRas-IP
(Plasmid
#13833)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMX-EGFP-gw-IP
- Backbone size w/o insert (bp) 6900
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRheb-HVR HRas
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Alt nameRheb
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SpeciesM. musculus (mouse)
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Insert Size (bp)700
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MutationN-terminal of Rheb and hyper variable region (HVR) of HRas are fused in frame
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Entrez GeneRheb (a.k.a. Rheb1)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-EGFP-Rheb-HVR HRas-IP was a gift from Shinya Yamanaka (Addgene plasmid # 13833 ; http://n2t.net/addgene:13833 ; RRID:Addgene_13833) -
For your References section:
Differential membrane localization of ERas and Rheb, two Ras-related proteins involved in the phosphatidylinositol 3-kinase/mTOR pathway. Takahashi K, Nakagawa M, Young SG, Yamanaka S. J Biol Chem. 2005 Sep 23. 280(38):32768-74. 10.1074/jbc.M506280200 PubMed 16046393
Map uploaded by the depositor.