pCF823_EFS-Cas9-PuroR_lenti
(Plasmid
#138317)
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PurposeLenti expression of SpyCas9 with Puro selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138317 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCF525
- Total vector size (bp) 10913
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpyCas9
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Insert Size (bp)4104
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCF823_EFS-Cas9-PuroR_lenti was a gift from Jennifer Doudna (Addgene plasmid # 138317 ; http://n2t.net/addgene:138317 ; RRID:Addgene_138317) -
For your References section:
Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. Watters KE, Shivram H, Fellmann C, Lew RJ, McMahon B, Doudna JA. Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117. 10.1073/pnas.1917668117 PubMed 32156733
Map uploaded by the depositor.
