pMXs-EGFP-ERas C220S/C222S-IP
(Plasmid #13829)

• Depositing Lab: Shinya Yamanaka
• Publication: Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-74.

Backbone

• Vector backbone: pMX-EGFP-gw-IP
• Backbone size w/o insert (bp): 6900
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERas C220S/C222S
• Species: M. musculus (mouse)
• Insert Size (bp): 700
• Mutation: C220 and C222 changed to serine residues
• Entrez Gene: Eras (a.k.a. RP23-198C2.4, HRAS2, HRasp, Ha-Ras2, ecat5)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry