pKEW397_AcrIIA15
(Plasmid
#138285)
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PurposeExpression of AcrIIA15 protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET
- Total vector size (bp) 6485
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcrIIA15
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SpeciesStaphylococcus delphini
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Tag
/ Fusion Protein
- MBP (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKEW397_AcrIIA15 was a gift from Jennifer Doudna (Addgene plasmid # 138285 ; http://n2t.net/addgene:138285 ; RRID:Addgene_138285) -
For your References section:
Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. Watters KE, Shivram H, Fellmann C, Lew RJ, McMahon B, Doudna JA. Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117. 10.1073/pnas.1917668117 PubMed 32156733
Map uploaded by the depositor.
