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pKEW215_AcrIIA13
(Plasmid #138281)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138281 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Total vector size (bp) 6531
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AcrIIA13
  • Species
    Staphylococcus schleiferi
  • GenBank ID
    WP_050337628.1
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKEW215_AcrIIA13 was a gift from Jennifer Doudna (Addgene plasmid # 138281 ; http://n2t.net/addgene:138281 ; RRID:Addgene_138281)

  • For your References section:

    Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. Watters KE, Shivram H, Fellmann C, Lew RJ, McMahon B, Doudna JA. Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117. 10.1073/pnas.1917668117 PubMed 32156733
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