pEF-AncBE4max
(Plasmid
#138270)
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PurposeExpression of AncBE4max-Cas9 Base-editor from an EF1a promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138270 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAncBE4max
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SpeciesSynthetic
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MutationD10A
- Promoter Ef1a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-AncBE4max was a gift from Xiao Wang (Addgene plasmid # 138270 ; http://n2t.net/addgene:138270 ; RRID:Addgene_138270) -
For your References section:
A transient reporter for editing enrichment (TREE) in human cells. Standage-Beier K, Tekel SJ, Brookhouser N, Schwarz G, Nguyen T, Wang X, Brafman DA. Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713. 10.1093/nar/gkz713 PubMed 31428784
