pcDNA3-FKBP-ICUE3-NLS
(Plasmid #138255)

• Purpose: FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
• Depositing Lab: Jin Zhang
• Publication: Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-891.e4. doi: 10.1016/j.chembiol.2019.03.003. Epub 2019 Apr 11.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5370
• Total vector size (bp): 9468
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FKBP-ICUE3-NLS
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 4098
• Promoter: CMV
• Tags / Fusion Proteins:
  • FKBP (N terminal on insert)
  • mECFP (N terminal on insert)
  • cpVenus[L194] (C terminal on insert)
  • Nuclear localization signal (NLS) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-FKBP-ICUE3-NLS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry