pcDNA3.1(+)-AKAP95-FRB
(Plasmid #138254)

• Purpose: Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.
• Depositing Lab: Jin Zhang
• Publication: Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-891.e4. doi: 10.1016/j.chembiol.2019.03.003. Epub 2019 Apr 11.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5366
• Total vector size (bp): 7772
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AKAP95-FRB
• Alt name: AKAP95
• Species: H. sapiens (human)
• Insert Size (bp): 2406
• Entrez Gene: AKAP8 (a.k.a. AKAP 95, AKAP-8, AKAP-95, AKAP95)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FRB rapamycin binding domain (C terminal on insert)
  • HA epitope (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3.1(+)-AKAP95-FRB
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry