pcDNA3-CBX1mut-PA-mCherry
(Plasmid #138251)

• Purpose: Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
• Depositing Lab: Jin Zhang
• Publication: Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi: 10.1126/sciadv.aau1447. eCollection 2018 Nov.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5523
• Total vector size (bp): 6414
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CBX1mut-PAmCherry
• Species: Synthetic
• Insert Size (bp): 891
• Promoter: CMV
• Tags / Fusion Proteins:
  • PAmCherry (C terminal on insert)
  • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-CBX1mut-PA-mCherry.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry