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Addgene

pFP.E227
(Plasmid #138249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB4A3
  • Backbone manufacturer
    BioBrick vector, IGEM registry
  • Backbone size w/o insert (bp) 3340
  • Total vector size (bp) 6206
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    729
  • Promoter iP16_3622
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer VF2 - TGCCACCTGACGTCTAAGAA
  • 3′ sequencing primer VR - ATTACCGCCTTTGAGTGAGC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Terminator
  • Alt name
    B0015
  • Species
    Synthetic
  • Insert Size (bp)
    129

Gene/Insert 3

  • Gene/Insert name
    mTagBFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter P17_up1691
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer VF2 - TGCCACCTGACGTCTAAGAA
  • 3′ sequencing primer VR - ATTACCGCCTTTGAGTGAGC
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    Terminator
  • Alt name
    B0015
  • Insert Size (bp)
    129

Gene/Insert 5

  • Gene/Insert name
    gfp
  • Alt name
    gfpmut3b
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • Promoter P20_992

Cloning Information for Gene/Insert 5

  • Cloning method Gibson Cloning
  • 5′ sequencing primer VF2 - TGCCACCTGACGTCTAAGAA
  • 3′ sequencing primer VR - ATTACCGCCTTTGAGTGAGC
    • (Common Sequencing Primers)

Gene/Insert 6

  • Gene/Insert name
    Terminator
  • Alt name
    B0015
  • Insert Size (bp)
    129

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFP.E227 was a gift from Baojun Wang (Addgene plasmid # 138249 ; http://n2t.net/addgene:138249 ; RRID:Addgene_138249)

  • For your References section:

    An expanded library of orthogonal split inteins enables modular multi-peptide assemblies. Pinto F, Thornton EL, Wang B. Nat Commun. 2020 Mar 23;11(1):1529. doi: 10.1038/s41467-020-15272-2. 10.1038/s41467-020-15272-2 PubMed 32251274
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