HDAC5 Flag
(Plasmid #13822)

• Purpose: Mammalian expression of human histone deacetylase 5 with flag tag
• Depositing Lab: Eric Verdin
• Publication: Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-20.

Backbone

• Vector backbone: pcDNA3.1 +
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HDAC5
• Alt name: HD5
• Alt name: NY-CO-9
• Species: H. sapiens (human)
• Insert Size (bp): 3366
• GenBank ID: NM_005474
• Entrez Gene: HDAC5 (a.k.a. HD5, NY-CO-9)
• Tag / Fusion Protein:
  • flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH Reverse

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).