pcDNA3-sACt-mCherry-NES
(Plasmid #138215)

• Purpose: Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.
• Depositing Lab: Jin Zhang
• Publication: Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82. doi: 10.1038/nchembio.799.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5499
• Total vector size (bp): 7656
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sACt-mCherry-NES
• Alt name: ADCY10
• Species: R. norvegicus (rat)
• Insert Size (bp): 2157
• Entrez Gene: Adcy10 (a.k.a. Sac)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (C terminal on insert)
  • Nuclear export signal (NES) (C terminal on insert)
  • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-sACt-mCherry-NES.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry